Description
Cholesterol is a naturally occurring sterol lipid that is a receiving growing emphasis in the bioprocessing space for its ability to improve DNA transfection efficiency by reducing the adherence of serum proteins to lipopolyplexes and increasing fusion with cellular membranes. The benefits of Cholesterol on cationic lipid complexes have been shown to increase transfection rates both in cell cultures and in vivo. Cholesterol has also been shown to reduce degradation of DNA from DNase in vivo. Cholesterol has been receiving increasing attention as a valuable component in forming lipid nanoparticles (LNPs) in the growing field of mRNA vaccines. LNPs facilitate the safe and effective delivery of mRNA to target cells, thus enabling therapeutic action. As of 2021, both Moderna and Pfizer-BioNTech COVID-19 vaccines utilize cholesterol as a critical part of their lipid formulations. Polysciences’ high purity, transfection grade Cholesterol can be used in conjunction with our other cationic lipid products to provide reliable performance for bioprocessing applications.